斑马鱼肾上腺素(EPI)酶联免疫吸附测定试剂盒

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货号

FY-EZe1645

样本类型

serum, plasma, cell lysates, cell culture supernates and other biological fluids

检测时间

1.5H

价格(美元)

280 400

规格

48T 96T

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DataSheet

说明书 MSDS

MSDS

产品详情

英文名称:

Zebrafish EPI(Epinephrine) ELISA Kit

种属:

Zebrafish

别称:

Adrenaline

检测方法:

Competitive

标准品:

2000 pg/mL

灵敏度:

9.74 pg/mL

检测范围:

31.25-2000 pg/mL

反应时间:

1.5H

样品类型:

serum, plasma, cell lysates, cell culture supernates and other biological fluids

研究领域:

Metabolic pathway;Infection immunity;Neuro science;Hormone metabolism;

测试原理:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with EPI protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to EPI. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EPI in the samples is then determined by comparing the OD of the samples to the standard curve.

技术数据

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration	OD1	OD2	Average OD
2000	0.187	0.188	0.188
1000	0.323	0.325	0.324
500	0.538	0.54	0.539
250	0.872	0.88	0.876
125	1.3	1.309	1.305
62.5	1.692	1.701	1.697
31.25	2.068	2.076	2.072
0	2.508	2.532	2.52

精密度

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.

Item	Intra-assay Precision	Intra-assay Precision
Sample	3	3
Replicate	9	18
CV(%)	5	8

回收率

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

样本类型	Range(%)	Average Recovery(%)
Serum (n=8)	87-97	90
EDTA plasma (n=8)	81-95	88
Cell culture media (n=8)	83-95	89

线性

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	95-103	85-94	82-90
1:2	Average (%)	96	89	88
1:4	Range (%)	86-92	85-94	85-92
1:4	Average (%)	90	94	91
1:8	Range (%)	89-97	80-96	80-96
1:8	Average (%)	92	95	95
1:16	Range (%)	91-103	92-101	92-101
1:16	Average (%)	91	98	96

检测流程图