Mouse CXCL15(Chemokine C-X-C-Motif Ligand 15) ELISA Kit

Mouse

C-X-C motif chemokine 15,Chemokine (C-X-C motif) ligand 15,Cxcl15,CXL15,Il8,Lungkine,Scyb15,Small inducible cytokine B15,Small inducible cytokine subfamily B member 15,Small-inducible cytokine B15,weche

Q9WVL7

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Metabolic pathway;Tumor immunity;Infection immunity;Developmental science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CXCL15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CXCL15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CXCL15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CXCL15 in the samples is then determined by comparing the OD of the samples to the standard curve.