Mouse RAB35(Ras-related protein Rab-35)    ELISA Kit

Mouse

Ras-related protein Rab-35,RAB35,GTP-binding protein RAY,Ras-related protein Rab-1C,RAB1C, RAY

Q6PHN9

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

CD & Adhesion molecule;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAB35. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RAB35. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAB35, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAB35 in the samples is then determined by comparing the OD of the samples to the standard curve.