Mouse RasGAP(Ras GTPase Activating Protein) ELISA Kit

Mouse

Ras GTPase-activating protein 1,RASA1,GAP,GTPase-activating protein,RasGAP,Ras p21 protein activator,p12GAP,GAP, RASA

F6SEU4 

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Infection immunity;Hematology;Developmental science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RasGAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RasGAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RasGAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RasGAP in the samples is then determined by comparing the OD of the samples to the standard curve.