Mouse CDKN1B(Cyclin Dependent Kinase Inhibitor 1B) ELISA Kit

Mouse

CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1, cyclin-dependent kinase inhibitor 1B, cyclin dependent kinase inhibitor 1B

P46414

Sandwich

500pg/mL

4.69 pg/mL

7.82-500pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CDKN1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CDKN1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CDKN1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CDKN1B in the samples is then determined by comparing the OD of the samples to the standard curve.