Mouse TAF2(Transcription initiation factor TFIID subunit 2) ELISA Kit

Mouse

Transcription initiation factor TFIID subunit 2,CIF15, MRT4, TAF2B, TAFII15,15 kDa cofactor of initiator function,RNA polymerase II TBP-associated factor subunit B,TBP-associated factor 15 kDa,Transcription initiation factor TFIID 15 kDa subunit,TAF(II)15,TAFII-15,TAFII15

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Metabolic pathway;Bone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TAF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TAF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TAF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TAF2 in the samples is then determined by comparing the OD of the samples to the standard curve.