Mouse HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

Mouse

HIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor

Q61221

Sandwich

8000pg/mL

75 pg/mL

125-8000pg/mL

3.5H

Tissue homogenate, Cell lysate

Signal transduction;Hematology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HIF-1α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HIF-1α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HIF-1α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HIF-1α in the samples is then determined by comparing the OD of the samples to the standard curve.