Mouse RANκ(Receptor Activator of Nuclear Factor Kappa B) ELISA Kit

Mouse

RANK, TNFRSF11A, CD265, TRANCER, FEO, LOH18CR1, ODFR, OFE, OPTB7, OSTS, PDB2, NFKB Activator

O35235

Sandwich

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RANκ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RANκ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RANκ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RANκ in the samples is then determined by comparing the OD of the samples to the standard curve.