Mouse PC4(activated RNA polymerase Ⅱ transcription cofactor 4) ELISA Kit

Mouse

SUB1, P15, PC4, p14, SUB1 homolog, transcriptional regulator, Activated RNA polymerase II transcriptional coactivator p15

P11031

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PC4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PC4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PC4 in the samples is then determined by comparing the OD of the samples to the standard curve.