Mouse EIF3A(Eukaryotic Translation Initiation Factor 3A) ELISA Kit

Mouse

EIF3A, EIF3, EIF3S1, P167, TIF32, eIF3-p17, eIF3-theta, p18, p185, eukaryotic translation initiation factor 3 subunit A

P23116

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EIF3A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to EIF3A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EIF3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EIF3A in the samples is then determined by comparing the OD of the samples to the standard curve.