Mouse SIGLEC12(Sialic Acid Binding Ig Like Lectin 12) ELISA Kit

Mouse

SIGLEC12, S2V, SIGLECL1, SLG, Siglec-XII, sialic acid binding Ig like lectin 12 (gene/pseudogene)

Q91Y57

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Apoptosis;Tumor immunity;Infection immunity;Immune molecule;Hematology;Reproductive science;Immunodeficiency;Autoimmunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SIGLEC12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SIGLEC12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SIGLEC12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SIGLEC12 in the samples is then determined by comparing the OD of the samples to the standard curve.