Mouse HSP-90(Heat Shock Protein 90) ELISA Kit

Mouse

Hsp9

P07901(Hsp90aa1)

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Apoptosis;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP-90. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HSP-90. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSP-90, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HSP-90 in the samples is then determined by comparing the OD of the samples to the standard curve.