Mouse PI3K2α(Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide) ELISA Kit

Mouse

Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing alpha polypeptide,PIK3C2A, CPK, PI3-K-C2(ALPHA), PI3-K-C2A, PI3K-C2-alpha, PI3K-C2alpha, phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha,Phosphoinositide 3-kinase-C2-alpha,PtdIns-3-kinase C2 subunit alpha

Q61194

Sandwich

100ng/mL

0.94 ng/mL

1.57-100ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PI3K2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PI3K2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PI3K2α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PI3K2α in the samples is then determined by comparing the OD of the samples to the standard curve.