Mouse PYGM(Glycogen Phosphorylase, Muscle) ELISA Kit

Mouse

GPMM, Phosphorylase,Glycogen, McArdle Syndrome,Glycogen Storage Disease Type V

Q9WUB3

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Cytokine;Signal transduction;Neuro science;Developmental science;Bone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PYGM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PYGM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PYGM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PYGM in the samples is then determined by comparing the OD of the samples to the standard curve.