Mouse α1-AT(Alpha 1-Antitrypsin) ELISA Kit

Mouse

SERPINA1, A1A, A1AT, AAT, PI, PI1, PRO2275

Sandwich

200ng/mL

1.88 ng/mL

3.13-200ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Endocrinology;Hormone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to α1-AT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to α1-AT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain α1-AT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of α1-AT in the samples is then determined by comparing the OD of the samples to the standard curve.