Mouse COMP(Cartilage Oligomeric Matrix Protein) ELISA Kit

Mouse

EDM1, EPD1, MED, PSACH, THBS5, TSP-5

Q9R0G6?

Sandwich

100ng/mL

0.94 ng/mL

1.57-100ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;CD & Adhesion molecule;Apoptosis;Tumor immunity;Infection immunity;Immune molecule;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to COMP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to COMP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COMP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of COMP in the samples is then determined by comparing the OD of the samples to the standard curve.