Mouse ApoH(Apolipoprotein H) ELISA Kit

Mouse

Apolipoprotein H,APOH, B2G1, B2GP1, BG,Beta-2-glycoprotein I,APC inhibitor,Beta(2) GPI,Apo-H,Anticardiolipin cofactor,Activated protein C-binding protein,

Q01339

Sandwich

500ng/mL

4.69 ng/mL

7.82-500ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Cell Biology

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ApoH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ApoH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ApoH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ApoH in the samples is then determined by comparing the OD of the samples to the standard curve.