Human PDGF-B(Platelet Derived Growth Factor Subunit B) ELISA Kit

Human

PDGF-2, PDGF2, SIS, SSV, c-sis

P01127

Sandwich

1500pg/mL

14.06 pg/mL

23.44-1500pg/mL

3.5H

serum, plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDGF-B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PDGF-B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDGF-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDGF-B in the samples is then determined by comparing the OD of the samples to the standard curve.