Human N-ERC/Mesothelin ELISA Kit

Human

N-ERC/MSLN, N-ERC/mesothelin

Q13421(37~286aa)

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;Metabolic pathway;Tumor immunity;Infection immunity;Cardiovascular biology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-ERC/Mesothelin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-ERC/Mesothelin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-ERC/Mesothelin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-ERC/Mesothelin in the samples is then determined by comparing the OD of the samples to the standard curve.