Human DAP6(Death Associated Protein 6) ELISA Kit

Human

DAXX, BING2, DAP6, EAP1, death-domain associated protein, death domain associated protein, Fas death domain-associated protein, ETS1-associated protein 1, hDaxx

Q9UER7

Sandwich

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Tumor immunity;Dermatology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DAP6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DAP6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DAP6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DAP6 in the samples is then determined by comparing the OD of the samples to the standard curve.