Human CD86(T-Lymphocyte Activation Antigen CD86) ELISA Kit

Human

CD86, B7-2, B7.2, B7, CD28LG2, LAB72, CD86 molecule, T-lymphocyte activation antigen CD86, Activation B7-2 antigen, CTLA-4 counter-receptor B7.2, FUN-1,

P42081

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Neuro science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD86. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CD86. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD86, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD86 in the samples is then determined by comparing the OD of the samples to the standard curve.