Human MBP-C/MBL2(Mannose Binding Protein C/Mannose Binding Lectin) ELISA Kit

Human

MBL2, COLEC1, HSMBPC, MBL, MBL2D, MBP, MBP-C, MBP1, MBPD

P11226

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Neuro science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MBP-C/MBL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MBP-C/MBL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MBP-C/MBL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MBP-C/MBL2 in the samples is then determined by comparing the OD of the samples to the standard curve.