Human TALLA-1/CD231(T-cell Acute Lymphoblastic Leukemia Antigen) ELISA Kit

Human

Tetraspanin-7,TSPAN7, A15, CCG-B7, CD231, DXS1692E, MRX58, MXS1, TM4SF2, TM4SF2b, tetraspanin 7,Tspan-7,Cell surface glycoprotein A15,

P41732

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Tumor immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TALLA-1/CD231. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TALLA-1/CD231. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TALLA-1/CD231, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TALLA-1/CD231 in the samples is then determined by comparing the OD of the samples to the standard curve.