Human PKM2(Pyruvate Kinase, Muscle) ELISA Kit

Human

PKM, CTHBP, HEL-S-3, OIP3, PK3, PKM2, TCB, THBP1, pyruvate kinase M1/2,Tumor M2-PK,p58

P14618

Sandwich

1000IU/mL

9.38 IU/mL

15.63-1000IU/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PKM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PKM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PKM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PKM2 in the samples is then determined by comparing the OD of the samples to the standard curve.