Human CD3g(Cluster of Differentiation 3g) ELISA Kit

Human

T-cell surface glycoprotein CD3 gamma chain,CD3G, CD3-GAMMA, IMD17, T3G, CD3g molecule,T-cell receptor T3 gamma chain,CD_antigen: CD3g

P09693

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Developmental science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD3g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CD3g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD3g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD3g in the samples is then determined by comparing the OD of the samples to the standard curve.