Human sFAS/sAPO-1(Soluble Factor-Related Apoptosis) ELISA Kit

Human

Apt1, Tnfrsf6, CD95, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, Tumor necrosis factor receptor superfamily member 6

P25445

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Developmental science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to sFAS/sAPO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to sFAS/sAPO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain sFAS/sAPO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of sFAS/sAPO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.