Human SIRT2(Sirtuin 2) ELISA Kit

Human

NAD-dependent protein deacetylase sirtuin-2,SIRT2,Regulatory protein SIR2 homolog 2,SIR2-like protein 2,SIR2L, SIR2L2,Sirtuin type 2

Q8IXJ6

Sandwich

50ng/mL

0.47 ng/mL

0.79-50ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Enzyme & Kinase;Metabolic pathway;Tumor immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SIRT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SIRT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SIRT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SIRT2 in the samples is then determined by comparing the OD of the samples to the standard curve.