Human GPS1(G Protein Pathway Suppressor 1) ELISA Kit

Human

COP9 signalosome complex subunit 1,COP9 signalosome subunit 1,COPS1,CSN 1,CSN1,G protein pathway suppressor 1,GPS-1,Gps1,JAB1-containing signalosome subunit 1,Protein GPS1,Protein MFH,SGN1,Signalosome subunit 1

Q13098

Sandwich

5000ng/mL

46.88 ng/mL

78.13-5000ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GPS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GPS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GPS1 in the samples is then determined by comparing the OD of the samples to the standard curve.