Human BANP(BTG3 Associated Nuclear Protein) ELISA Kit

Human

Protein BANP,BEND1, SMAR1, SMARBP1,BEN domain-containing protein 1,Scaffold/matrix-associated region-1-binding protein

Q8N9N5

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Hematology;Cardiovascular biology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to BANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BANP in the samples is then determined by comparing the OD of the samples to the standard curve.