Human Ntn4(Netrin 4) ELISA Kit

Human

Beta-netrin, Hepar-derived netrin-like protein

Q9HB63

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Cytokine;Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ntn4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ntn4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ntn4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ntn4 in the samples is then determined by comparing the OD of the samples to the standard curve.