Human NFYC(Nuclear Transcription Factor Y Gamma) ELISA Kit

Human

NFYC, CBF-C, CBFC, H1TF2A, HAP5, HSM, NF-YC, nuclear transcription factor Y subunit gamma

Q13952

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Cytokine;Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFYC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NFYC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NFYC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NFYC in the samples is then determined by comparing the OD of the samples to the standard curve.