Human PTPN6(Protein Tyrosine Phosphatase, Non Receptor Type 6) ELISA Kit

Human

HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, Src homology region 2 domain-containing phosphatase-1

P29350

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Tissue homogenate and Cell lysate

Cytokine;Tumor immunity;Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTPN6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PTPN6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PTPN6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PTPN6 in the samples is then determined by comparing the OD of the samples to the standard curve.