Human PTPN11(Protein Tyrosine Phosphatase, Non Receptor Type 11) ELISA Kit

Human

BPTP3, CFC, NS1, PTP-1D, PTP2-C, SH-PTP2, SH-PTP3, SHP2

Q06124

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Tissue homogenate and Cell lysate

Tumor immunity;Endocrinology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTPN11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PTPN11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PTPN11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PTPN11 in the samples is then determined by comparing the OD of the samples to the standard curve.