Human PAI2(Plasminogen Activator Inhibitor 2) ELISA Kit

Human

SERPINB2, HsT121, PAI, PAI-2, PAI2, PLANH2, serpin family B member 2, Monocyte Arg-serpin, Placental plasminogen activator inhibitor, Serpin B2, Urokinase inhibitor

P05120

Sandwich

100ng/mL

0.94 ng/mL

1.57-100ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Metabolic pathway;Bone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PAI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PAI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PAI2 in the samples is then determined by comparing the OD of the samples to the standard curve.