Human GSK3α(Glycogen Synthase Kinase 3 Alpha) ELISA Kit

Human

GSK3A, Gsk3a, 2786H6Rik, glycogen synthase kinase 3 alpha,Glycogen synthase kinase-3 alpha,GSK-3 alpha,Serine/threonine-protein kinase GSK3A

P49840

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Endocrinology;Hormone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GSK3α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GSK3α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GSK3α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GSK3α in the samples is then determined by comparing the OD of the samples to the standard curve.