Human GRβ(Glucocorticoid Receptor Beta) ELISA Kit

Human

GCCR,GCR,glucocorticoid nuclear receptor variant 1,Glucocorticoid receptor,Glucocorticoid receptor beta isoform,GR,GRL,nr3c1,Nuclear receptor subfamily 3 group C member 1

P04150

Sandwich

5000pg/mL

46.88 pg/mL

78.13-5000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Developmental science;Bone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRβ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GRβ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GRβ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GRβ in the samples is then determined by comparing the OD of the samples to the standard curve.