Human ECFR(Eosinophil Chemotactic Factor Receptor) ELISA Kit

Human

CCR3, CC-CKR-3, CD193, CKR3, CMKBR3, C-C motif chemokine receptor 3,C-C chemokine receptor type 3,C-C CKR-3,Eosinophil eotaxin receptor,CD_antigen: CD193,cluster of differentiation 193

P51677

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Infection immunity;Neuro science;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ECFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ECFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ECFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ECFR in the samples is then determined by comparing the OD of the samples to the standard curve.