Human PSG1/SP1(Pregnancy Specific Beta-1-Glycoprotein 1) ELISA Kit

Human

Pregnancy specific beta-1-glycoprotein 1 ,PSBG-1,CD66f ,Cluster of Differentiation 66f,PSG1, B1G1, CD66f, DHFRP2, FL-NCA-1/2, PBG1, PS-beta-C/D, PS-beta-G-1, PSBG-1, PSBG1, PSG95, PSGGA, PSGIIA, SP1

P11464

Sandwich

200ng/mL

1.88 ng/mL

3.13-200ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Cardiovascular biology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSG1/SP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PSG1/SP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PSG1/SP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PSG1/SP1 in the samples is then determined by comparing the OD of the samples to the standard curve.