Human PT(Prothrombin) ELISA Kit

Human

Prothrombin,coagulation factor II, F2, PT, RPRGL2, THPH1

P00734

Sandwich

600ng/mL

5.63 ng/mL

9.38-600ng/mL

3.5H

Plasma, Becomes thrombin in Serum

Enzyme & Kinase;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PT in the samples is then determined by comparing the OD of the samples to the standard curve.