Human PKCη(Protein Kinase C Eta) ELISA Kit

Human

PRKCH, PKC-L, PKCL, PRKCL, nPKC-eta, protein kinase C eta

P24723

Sandwich

200mIU/mL

1.88 mIU/mL

3.13-200mIU/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;Tumor immunity;Developmental science;Bone metabolism;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PKCη. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PKCη. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PKCη, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PKCη in the samples is then determined by comparing the OD of the samples to the standard curve.