Human PDGF-AB(Platelet Derived Growth Factor AB) ELISA Kit

Human

PDGF-AB

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Immune molecule;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDGF-AB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PDGF-AB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDGF-AB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDGF-AB in the samples is then determined by comparing the OD of the samples to the standard curve.