Human MCSFR(Macrophage Colony Stimulating Factor Receptor) ELISA Kit

Human

CSF1R, Colony stimulating factor 1 receptor, CD115, C-FMS, CSF-1R, FIM2, FMS, M-CSFR

P07333

Sandwich

4000pg/mL

37.5 pg/mL

62.5-4000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Endocrinology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MCSFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MCSFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MCSFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MCSFR in the samples is then determined by comparing the OD of the samples to the standard curve.