Human HLTF(Helicase Like Transcription Factor) ELISA Kit

Human

HLTF, HIP116, HIP116A, HLTF1, RNF8, SMARCA3, SNF2L3, ZBU1, helicase-like transcription factor, helicase like transcription factor

Q14527

Sandwich

2000pg/mL

18.75 pg/mL

31.25-2000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Infection immunity;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HLTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HLTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HLTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HLTF in the samples is then determined by comparing the OD of the samples to the standard curve.