Human Sec23b(Protein transport protein Sec23B) ELISA Kit

Human

SEC23B, CDA-II, CDAII, CDAN2, HEMPAS, CWS7, Sec23 homolog B, coat complex II component, hSec23B,SEC23-related protein B

Q15437

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sec23b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sec23b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sec23b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sec23b in the samples is then determined by comparing the OD of the samples to the standard curve.