Human RPL10(Ribosomal Protein L10) ELISA Kit

Human

RPL1, AUTSX5, DXS648, DXS648E, L1, NOV, QM, ribosomal protein L1, MRXS35

P27635

Sandwich

40ng/mL

0.38 ng/mL

0.63-40ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RPL10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to RPL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RPL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RPL10 in the samples is then determined by comparing the OD of the samples to the standard curve.