Human NUP205(Nucleoporin 205kDa) ELISA Kit

Human

C7orf14, NPHS13, nucleoporin 25,Nuclear pore complex protein Nup25,25 kDa nucleoporin,Nucleoporin Nup25,C7orf14, KIAA225

Q92621

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Signal transduction;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NUP205. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NUP205. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NUP205, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NUP205 in the samples is then determined by comparing the OD of the samples to the standard curve.