Human SIGLEC10(Sialic Acid Binding Immunoglobulin Like Lectin 10) ELISA Kit

Human

mSiglec G,PRO94,Sialic acid binding Ig like lectin 1,Sialic acid binding Ig like lectin 1,sialic acid binding Ig like lectin 1 Ig like lectin 7,sialic acid binding Ig like lectin G,Sialic acid binding Immunoglobulin like lectin 1,Sialic acid-binding Ig-like lectin 1,SIG1,SIGLEC 1,Siglec G,siglec like gene 2,Siglec like protein 2,Siglec like protein 2,Siglec-1,Siglec-like protein 2,SIGLEC1,Siglecg,SLG2

Q96LC7

Sandwich

10ng/mL

0.1 ng/mL

0.16-10ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SIGLEC10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SIGLEC10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SIGLEC10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SIGLEC10 in the samples is then determined by comparing the OD of the samples to the standard curve.