Human GP-73(Golgi Protein 73) ELISA Kit

Human

GP73, GOLM1, Golgi Membrane Protein 1, GOLPH2, Golgi Phosphoprotein 2, C9orf155, bA379P1.3, PSEC257

Q8NBJ4

Sandwich

40ng/mL

0.38 ng/mL

0.63-40ng/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Enzyme & Kinase;Metabolic pathway;Hematology;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GP-73. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to GP-73. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GP-73, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GP-73 in the samples is then determined by comparing the OD of the samples to the standard curve.