Human REG3α(Regenerating Islet Derived Protein 3 Alpha) ELISA Kit

Human

INGAP,HIP, PAP, PAP-H,PAP1,PBCGF,REG-Ⅲ,REG3, PAP homologous protein,hepatocarcinoma-intestine-pancreas,pancreatic beta cell growth factor,pancreatitis-associated protein,proliferation-inducing prote

Q06141

Sandwich

1000pg/mL

9.38 pg/mL

15.63-1000pg/mL

3.5H

Serum, Plasma, Tissue homogenate and Other biological samples

Immune molecule;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to REG3α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to REG3α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain REG3α, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of REG3α in the samples is then determined by comparing the OD of the samples to the standard curve.