Human SPC(Pulmonary Surfactant Associated Protein C) ELISA Kit

Human

SFTPC , BRICD6, PSP-C, SFTP2, SMDP2

P11686

Sandwich

20ng/mL

0.19 ng/mL

0.32-20ng/mL

3.5H

Serum, Tissue homogenate, Cell lysate, Cell culture supernatant, Alveolar lavage fluid(Serum, tissuehomogenates, celllysates, cellculturesupernates, lunglavagefluid)

Enzyme & Kinase;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to SPC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPC in the samples is then determined by comparing the OD of the samples to the standard curve.